Increase your confidence in the data
you obtain from your RNAi experiments with these
ten recommendations. Example: Allow for at least 2 nucleotide mismatches with all off-target genes. [go]
Technical
Bulletin #506: siRNA Design Guidelines
Learn how to select the best siRNA target
site, design effective siRNAs, and create vector inserts
that generate optimal hairpin siRNAs by reading these recommendations
derived from the literature as well as from Ambion scientists'
first-hand experience. [go]