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Calculator for Suspension of Dry Single-stranded Oligonucleotides

Instructions
Oligonucleotides are often supplied dry. The specification sheet provided by oligonucleotide manufacturers often contains the following information:

  • nmoles synthesized
  • mass amount synthesized
  • OD260 units synthesized

When provided with one of the above pieces of information and either the sequence or the length of the oligonucleotide, this calculator will determine the molecular weight of the oligonucleotide and how much water or buffer to add to make a solution of the desired concentration.

The calculations done by this calculator are outlined on the following web page and in the Silencer siRNA Labeling Kit Instruction Manual.

Oligonucleotide type: RNA* DNA
*Including those with 3' TT ends.

Concentration of stock solution desired in µM
(use 50 µM for the Silencer siRNA Labeling Kit):
µM

What units of dry oligonucleotide do you wish to provide?



Related Articles
Base:Dye Ratio Calculator

Silencer® siRNA Labeling Kit Instruction Manual


Ordering Information
For prices and availability, please contact our Customer Service Department.

Cat# Product Name Size
AM1632 Silencer® siRNA Labeling Kit - Cy™3 65 µg labeled siRNA
AM1634 Silencer® siRNA Labeling Kit - FAM™ 65 µg labeled siRNA

 
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