Ambion's Appendix - RNA and DNA Concentration Converter

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RNA and DNA Concentration Converter

For Converting ng/ml to pM or Vice Versa

Instructions
Fill in answers to 1–3 and either 4 (for converting ng/ml to pM) or 5 (for converting pM to ng/ml).

1. Convert:

ng/ml to pM

pM to ng/ml

2. Sample Type:

RNA

ssDNA

dsDNA

3. Length (nt):

4. Concentration (ng/ml):

5. Concentration (pM):

Notes About Calculation

Approximate MW of Nucleic Acids
MW of ssRNA = (length in nt x 320.5) + 159.0
MW of ssDNA = (length in nt x 303.7) + 79.0
MW of dsDNA = (length in nt x 607.4) + 157.9

Conversions
pM = ((ng/ml) x 10⁶) / MW
ng/ml = (pM x MW) / 10⁶